UNIT 17.18 Analysis of Monosaccharides
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Manzi, A. E., Powell, L. D. and Varki, A. 2001. Analysis of Monosaccharides. Current Protocols in Molecular Biology. 32:IV:17.18:17.18.1–17.18.19.
- Published Online: 1 MAY 2001
- Published Print: OCT 1995
This unit presents methods for assaying sialic acids, reducing sugars, and hexosamines. The BCA assay detects free reducing terminii in sugars released from glycoconjugates by appropriate treatments. Assays employing Ehrlich reagent (DMAB) detect hexosamines and N-acetylhexosamines, including a method for hydrolyzing the glycosidic linkages of the hexosamines and a method for re-N-acetylation. The TBA and DMB assays can be used to quantitate and fractionate free forms of many types of sialic acids. Techniques for liberating the sialic acids from the parent glycoconjugates are also provided.