Unit

UNIT 17.18 Analysis of Monosaccharides

  1. Adriana E. Manzi,
  2. Leland D. Powell,
  3. Ajit Varki (thiobarbituric acid assay; de-O-acetylation)

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1718s32

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Manzi, A. E., Powell, L. D. and Varki, A. 2001. Analysis of Monosaccharides. Current Protocols in Molecular Biology. 32:IV:17.18:17.18.1–17.18.19.

Author Information

  1. University of California San Diego, La Jolla, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1995

Abstract

This unit presents methods for assaying sialic acids, reducing sugars, and hexosamines. The BCA assay detects free reducing terminii in sugars released from glycoconjugates by appropriate treatments. Assays employing Ehrlich reagent (DMAB) detect hexosamines and N-acetylhexosamines, including a method for hydrolyzing the glycosidic linkages of the hexosamines and a method for re-N-acetylation. The TBA and DMB assays can be used to quantitate and fractionate free forms of many types of sialic acids. Techniques for liberating the sialic acids from the parent glycoconjugates are also provided.