UNIT 17.21A HPLC Methods for the Fractionation and Analysis of Negatively Charged Oligosaccharides and Gangliosides
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Manzi, A. E. and Hayes, B. K. 2001. HPLC Methods for the Fractionation and Analysis of Negatively Charged Oligosaccharides and Gangliosides. Current Protocols in Molecular Biology. 32:IV:17.21A:17.21.1–17.21.8.
- Published Online: 1 MAY 2001
- Published Print: OCT 1995
This unit describes the fractionation and analysis of anionic oligosaccharides and gangliosides using anion-exchange high-performance liquid chromatography (HPLC). Saccharides or gangliosides are eluted in order of the number of negative charges they possess, although the charge-to-mass ratio can also contribute to elution position.