Unit

UNIT 17.21A HPLC Methods for the Fractionation and Analysis of Negatively Charged Oligosaccharides and Gangliosides

  1. Adriana E. Manzi1,
  2. Bradley K. Hayes2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1721as32

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Manzi, A. E. and Hayes, B. K. 2001. HPLC Methods for the Fractionation and Analysis of Negatively Charged Oligosaccharides and Gangliosides. Current Protocols in Molecular Biology. 32:IV:17.21A:17.21.1–17.21.8.

Author Information

  1. 1

    University of California San Diego, La Jolla, California

  2. 2

    University of Alabama at Birmingham, Birmingham, Alabama

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1995

Abstract

This unit describes the fractionation and analysis of anionic oligosaccharides and gangliosides using anion-exchange high-performance liquid chromatography (HPLC). Saccharides or gangliosides are eluted in order of the number of negative charges they possess, although the charge-to-mass ratio can also contribute to elution position.