UNIT 17.21B Fractionation and Analysis of Neutral Oligosaccharides by HPLC
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Hayes, B. K. and Varki, A. 2001. Fractionation and Analysis of Neutral Oligosaccharides by HPLC. Current Protocols in Molecular Biology. 32:IV:17.21B:17.21.9–17.21.13.
- Published Online: 1 MAY 2001
- Published Print: OCT 1995
This unit describes the fractionation and analysis of neutral oligosaccharides by high-performance liquid chromatography (HPLC) on bonded amine columns. Separation is based upon hydrogen bonding between the NH2 groups of the column and the hydroxyl groups of the oligosaccharides. A support protocol describes the reduction and desalting of neutral oligosaccharides with sodium borohydride.