Unit

UNIT 17.21B Fractionation and Analysis of Neutral Oligosaccharides by HPLC

  1. Bradley K. Hayes1,
  2. Ajit Varki2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1721bs32

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Hayes, B. K. and Varki, A. 2001. Fractionation and Analysis of Neutral Oligosaccharides by HPLC. Current Protocols in Molecular Biology. 32:IV:17.21B:17.21.9–17.21.13.

Author Information

  1. 1

    University of Alabama at Birmingham, Birmingham, Alabama

  2. 2

    University of California Sun Diego, La Jolla, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1995

Abstract

This unit describes the fractionation and analysis of neutral oligosaccharides by high-performance liquid chromatography (HPLC) on bonded amine columns. Separation is based upon hydrogen bonding between the NH2 groups of the column and the hydroxyl groups of the oligosaccharides. A support protocol describes the reduction and desalting of neutral oligosaccharides with sodium borohydride.