Unit

UNIT 17.22B Analysis of Disaccharides and Tetrasaccharides Released from Glycosaminoglycans

  1. H. Edward Conrad

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1722bs32

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Conrad, H. E. 2001. Analysis of Disaccharides and Tetrasaccharides Released from Glycosaminoglycans. Current Protocols in Molecular Biology. 32:IV:17.22B:17.20.1–17.20.7.

Author Information

  1. University of Illinois, Urbana, Illinois

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1995

Abstract

Glycosaminoglycans (GAGs) are converted to disaccharides by various methods. This unit describes separation of individual disaccharides by paper chromatography or paper electrophoresis or high-performance liquid chromatography (HPLC). Lyase-released disaccharides can also be monitored by UV absorbance. Support protocols describe mild conditions for reduction of alkali-labile disaccharides obtained by cleavage of GAGs with lyases, scintillation counting of samples obtained from HPLC separation of radiolabeled saccharides, and calculations for the quantitation of disaccharides.