UNIT 17.22B Analysis of Disaccharides and Tetrasaccharides Released from Glycosaminoglycans
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Conrad, H. E. 2001. Analysis of Disaccharides and Tetrasaccharides Released from Glycosaminoglycans. Current Protocols in Molecular Biology. 32:IV:17.22B:17.20.1–17.20.7.
- Published Online: 1 MAY 2001
- Published Print: OCT 1995
Glycosaminoglycans (GAGs) are converted to disaccharides by various methods. This unit describes separation of individual disaccharides by paper chromatography or paper electrophoresis or high-performance liquid chromatography (HPLC). Lyase-released disaccharides can also be monitored by UV absorbance. Support protocols describe mild conditions for reduction of alkali-labile disaccharides obtained by cleavage of GAGs with lyases, scintillation counting of samples obtained from HPLC separation of radiolabeled saccharides, and calculations for the quantitation of disaccharides.