UNIT 17.23 Analysis of Sulfate Esters by Solvolysis or Hydrolysis
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Freeze, H. H. 2001. Analysis of Sulfate Esters by Solvolysis or Hydrolysis. Current Protocols in Molecular Biology. 32:17.23.1–17.23.8.
- Published Online: 1 MAY 2001
- Published Print: OCT 1995
Sulfate esters are found on N- and O-linked sugar chains or glycosaminoglycan (GAG) chains. Few sulfatases are available that can enzymatically remove them, so chemical procedures must be used. These procedures rely on the differential sensitivity of sulfates located in different linkages on the sugar. In comparison to the conditions used for enzymatic digestion, those used for chemical digestion are very harsh and cannot be used on protein-bound carbohydrates (except for analytical purposes, as described here). With protein-bound carbohydrates, for preparative purposes, the chains must first be released. This unit describes release of sulfate esters by solvolysis along with a method for monitoring the efficiency of the solvolysis reaction. An alternate procedure provides a scale-up method for using the solvolysis reaction with large amounts of material. Also presented are techniques for both acid and basic hydrolysis to release sulfate esters.