Unit

UNIT 17.23 Analysis of Sulfate Esters by Solvolysis or Hydrolysis

  1. Hudson H. Freeze

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1723s32

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Freeze, H. H. 2001. Analysis of Sulfate Esters by Solvolysis or Hydrolysis. Current Protocols in Molecular Biology. 32:17.23.1–17.23.8.

Author Information

  1. La Jolla Cancer Research Foundation, La Jolla, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1995

Abstract

Sulfate esters are found on N- and O-linked sugar chains or glycosaminoglycan (GAG) chains. Few sulfatases are available that can enzymatically remove them, so chemical procedures must be used. These procedures rely on the differential sensitivity of sulfates located in different linkages on the sugar. In comparison to the conditions used for enzymatic digestion, those used for chemical digestion are very harsh and cannot be used on protein-bound carbohydrates (except for analytical purposes, as described here). With protein-bound carbohydrates, for preparative purposes, the chains must first be released. This unit describes release of sulfate esters by solvolysis along with a method for monitoring the efficiency of the solvolysis reaction. An alternate procedure provides a scale-up method for using the solvolysis reaction with large amounts of material. Also presented are techniques for both acid and basic hydrolysis to release sulfate esters.