UNIT 18.7 Assays of Protein Kinases Using Exogenous Substrates
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Carter, A. N. 2001. Assays of Protein Kinases Using Exogenous Substrates. Current Protocols in Molecular Biology. 40:18.7:18.7.1–18.7.22.
- Published Online: 1 MAY 2001
- Published Print: OCT 1997
In studies of the regulation of specific biochemical events by reversible phosphorylation, assaying the protein kinases themselves can often lead to significant progress in understanding the mechanistic details of a system under study. This unit describes assays for a variety of protein kinases that require different conditions to detect and measure their activities: cyclic nucleotide-dependent kinases, protein kinase C and isoforms, casein kinases, Ca2+/calmodulin-dependent kinases, and tyrosine kinase. A protocol for in-gel assays for specific kinase activity is also provided. The unit is not meant to be a catalog of individual protein kinase assays; however, the general principles of these assays should apply to most if not all known protein kinases.