UNIT 18.12 Detection of MAP Kinase Signaling
Published Online: 1 SEP 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Rubinfeld, H. and Seger, R. 2004. Detection of MAP Kinase Signaling. Current Protocols in Molecular Biology. 18:18.12.
- Published Online: 1 SEP 2004
- Published Print: JUL 2004
This is not the most recent version of the article. View current version (1 FEB 2006)
Elucidation of intracellular signaling pathway is essential to understanding the regulatory mechanisms of cellular function. Mitogen-activated protein kinase (MAPK) signaling cascades are a major pathway in intracellular signaling. The methods used to detect cascade activation involve measurements of protein kinase activities. This unit describes an assay for specific immunoprecipitation of a protein kinase and detection of phosphorylation of the appropriate substrate, an in-gel kinase assay for kinases of unknown identity or for which there are no specific antibodies, and the JNK assay, which involves a broad-based immunoaffinity purification followed by phosphorylation.