Unit

UNIT 18.12 Detection of MAP Kinase Signaling

  1. Hadara Rubinfeld,
  2. Rony Seger

Published Online: 1 SEP 2004

DOI: 10.1002/0471142727.mb1812s67

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Rubinfeld, H. and Seger, R. 2004. Detection of MAP Kinase Signaling. Current Protocols in Molecular Biology. 18:18.12.

Author Information

  1. The Weizmann Institute of Science, Rehovot, Israel

Publication History

  1. Published Online: 1 SEP 2004
  2. Published Print: JUL 2004

This is not the most recent version of the article. View current version (1 FEB 2006)

Abstract

Elucidation of intracellular signaling pathway is essential to understanding the regulatory mechanisms of cellular function. Mitogen-activated protein kinase (MAPK) signaling cascades are a major pathway in intracellular signaling. The methods used to detect cascade activation involve measurements of protein kinase activities. This unit describes an assay for specific immunoprecipitation of a protein kinase and detection of phosphorylation of the appropriate substrate, an in-gel kinase assay for kinases of unknown identity or for which there are no specific antibodies, and the JNK assay, which involves a broad-based immunoaffinity purification followed by phosphorylation.