Unit

UNIT 18.15 Visualization of Kinase Activity with FRET-Based Activity Biosensors

  1. Charlene Depry1,
  2. Jin Zhang1,2

Published Online: 1 JUL 2010

DOI: 10.1002/0471142727.mb1815s91

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Depry, C. and Zhang, J. 2010. Visualization of Kinase Activity with FRET-Based Activity Biosensors. Current Protocols in Molecular Biology. 91:18.15.1–18.15.9.

Author Information

  1. 1

    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland

  2. 2

    The Solomon H. Snyder Department of Neuroscience and Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, Maryland

Publication History

  1. Published Online: 1 JUL 2010
  2. Published Print: JUL 2010

Abstract

Genetically encodable FRET-based kinase activity reporters (KARs) enable real-time monitoring of kinase activity dynamics in living cells with high spatiotemporal resolution. This unit describes a general protocol for utilizing KARs to visualize kinase activity in living mammalian cells with fluorescence microscopy. Curr. Protoc. Mol. Biol. 91:18.15.1-18.15.9. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • kinase activity reporter;
  • FRET;
  • live-cell imaging