UNIT 18.15 Visualization of Kinase Activity with FRET-Based Activity Biosensors
Published Online: 1 JUL 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Depry, C. and Zhang, J. 2010. Visualization of Kinase Activity with FRET-Based Activity Biosensors. Current Protocols in Molecular Biology. 91:18.15:18.15.1–18.15.9.
- Published Online: 1 JUL 2010
- Published Print: JUL 2010
Genetically encodable FRET-based kinase activity reporters (KARs) enable real-time monitoring of kinase activity dynamics in living cells with high spatiotemporal resolution. This unit describes a general protocol for utilizing KARs to visualize kinase activity in living mammalian cells with fluorescence microscopy. Curr. Protoc. Mol. Biol. 91:18.15.1-18.15.9. © 2010 by John Wiley & Sons, Inc.
- kinase activity reporter;
- live-cell imaging