UNIT 18.18 Serine/Threonine Protein Phosphatase Assays
Published Online: 1 OCT 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
McAvoy, T. and Nairn, A. C. 2010. Serine/Threonine Protein Phosphatase Assays. Current Protocols in Molecular Biology. 92:18.18:18.18.1–18.18.11.
- Published Online: 1 OCT 2010
- Published Print: OCT 2010
Methods for assaying serine/threonine protein phosphatases are discussed. Three commonly used protocols are presented that employ either colorimetric or radiometric assays. These methods can be used to assay a variety of preparations of serine/threonine phosphatases, from crude lysates to purified proteins. Strategies for the application of a particular protocol for a particular purpose are discussed. The assays can be used in either a high-throughput mode where simple comparison of activities can be done, or in specific assays where kinetic data can be derived. Curr. Protoc. Mol. Biol. 92:18.18.1-18.18.11. © 2010 by John Wiley & Sons, Inc.
- protein phosphorylation;
- protein phosphatase;
- colorimetric assay;
- radiometric assay