UNIT 19.3 Sequence Similarity Searching Using the BLAST Family of Programs
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Wolfsberg, T. G. and Madden, T. L. 2001. Sequence Similarity Searching Using the BLAST Family of Programs. Current Protocols in Molecular Biology. 46:19.3:19.3.1–19.3.29.
- Published Online: 1 MAY 2001
- Published Print: APR 1999
Database sequence similarity searching is carried out thousands of times each day by researchers worldwide and has become a very valuable tool. Over the years, a number of algorithms have been implemented to facilitate database searching. The BLAST (Basic Local Alignment Research Tool) family of sequence similarity search programs allows searches to be done quickly and easily, but with sensitive, yet rigorous statistical expectations. In this unit, which is a completely new version of its predecessor of the same title, the user learns how to access the databases, determine the correct searching strategies, and apply examples of BLAST searches to his or her own data.