UNIT 19.7 Using PATIMDB to Create Bacterial Transposon Insertion Mutant Libraries
Published Online: 1 APR 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Urbach, J. M., Wei, T., Liberati, N., Grenfell-Lee, D., Villanueva, J., Wu, G. and Ausubel, F. M. 2009. Using PATIMDB to Create Bacterial Transposon Insertion Mutant Libraries. Current Protocols in Molecular Biology. 86:19.7:19.7.1–19.7.34.
- Published Online: 1 APR 2009
- Published Print: APR 2009
PATIMDB is a software package for facilitating the generation of transposon mutant insertion libraries. The software has two main functions: process tracking and automated sequence analysis. The process tracking function specifically includes recording the status and fates of multiwell plates and samples in various stages of library construction. Automated sequence analysis refers specifically to the pipeline of sequence analysis starting with ABI files from a sequencing facility and ending with insertion location identifications. The protocols in this unit describe installation and use of PATIMDB software. Curr. Protoc. Mol. Biol. 86:19.7.1-19.7.34. © 2009 by John Wiley & Sons, Inc.
- process tracking;