UNIT 20.1 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins

  1. Erica A. Golemis1,
  2. Ilya Serebriiskii1,
  3. Russell L. Finley Jr.2,
  4. Mikhail G. Kolonin (hunt by interaction mating)2,
  5. Jeno Gyuris3,
  6. Roger Brent4

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb2001s46

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Golemis, E. A., Serebriiskii, I., Finley, R. L., Kolonin (hunt by interaction mating), M. G., Gyuris, J. and Brent, R. 2001. Interaction Trap/Two-Hybrid System to Identify Interacting Proteins. Current Protocols in Molecular Biology. 46:20.1:20.1.1–20.1.40.

Author Information

  1. 1

    Fox Chase Cancer Center, Philadelphia, Pennsylvania

  2. 2

    Wayne State University School of Medicine, Detroit, Michigan

  3. 3

    Mitotix, Inc., Cambridge, Massachusetts

  4. 4

    The Molecular Sciences Institute, Berkeley, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1999

This is not the most recent version of the article. View current version (1 APR 2008)


The parameters and experimental conditions for this important system are constantly undergoing improvement. This newest version includes expanded tables describing interaction trap components and additional libraries compatible with the interaction trap system. It also features a new protocol on performing a hunt by interaction mating. Some of the commercial vendors selling yeast two-hybrid reagents recommend using interaction mating to perform a hunt, so this procedure should be of great interest.