UNIT 20.1 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Golemis, E. A., Serebriiskii, I., Finley, R. L., Kolonin (hunt by interaction mating), M. G., Gyuris, J. and Brent, R. 2001. Interaction Trap/Two-Hybrid System to Identify Interacting Proteins. Current Protocols in Molecular Biology. 46:20.1:20.1.1–20.1.40.
- Published Online: 1 MAY 2001
- Published Print: APR 1999
This is not the most recent version of the article. View current version (1 APR 2008)
The parameters and experimental conditions for this important system are constantly undergoing improvement. This newest version includes expanded tables describing interaction trap components and additional libraries compatible with the interaction trap system. It also features a new protocol on performing a hunt by interaction mating. Some of the commercial vendors selling yeast two-hybrid reagents recommend using interaction mating to perform a hunt, so this procedure should be of great interest.