UNIT 20.1 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
Published Online: 1 APR 2008
Copyright © 2008 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Golemis, E. A., Serebriiskii, I., Finley, R. L., Kolonin (hunt by interaction mating), M. G., Gyuris, J. and Brent, R. 2008. Interaction Trap/Two-Hybrid System to Identify Interacting Proteins. Current Protocols in Molecular Biology. 82:20.1:20.1.1–20.1.35.
- Published Online: 1 APR 2008
- Published Print: APR 2008
The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins. Curr. Protoc. Mol. Biol. 82:20.1.1-20.1.35. © 2008 by John Wiley & Sons, Inc.
- protein interactions;
- yeast two-hybrid;
- interaction trap;
- interaction mating