Unit

UNIT 20.1 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins

  1. Erica A. Golemis1,
  2. Ilya Serebriiskii1,
  3. Russell L. Finley Jr. (hunt by interaction mating)2,
  4. Mikhail G. Kolonin (hunt by interaction mating)2,
  5. Jeno Gyuris3,
  6. Roger Brent4

Published Online: 1 APR 2008

DOI: 10.1002/0471142727.mb2001s82

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Golemis, E. A., Serebriiskii, I., Finley, R. L., Kolonin (hunt by interaction mating), M. G., Gyuris, J. and Brent, R. 2008. Interaction Trap/Two-Hybrid System to Identify Interacting Proteins. Current Protocols in Molecular Biology. 82:20.1:20.1.1–20.1.35.

Author Information

  1. 1

    Fox Chase Cancer Center, Philadelphia, Pennsylvania

  2. 2

    Wayne State University School of Medicine, Detroit, Michigan

  3. 3

    Mitotix, Inc., Cambridge, Massachusetts

  4. 4

    The Molecular Sciences Institute, Berkeley, California

Publication History

  1. Published Online: 1 APR 2008
  2. Published Print: APR 2008

Abstract

The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins. Curr. Protoc. Mol. Biol. 82:20.1.1-20.1.35. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • protein interactions;
  • yeast two-hybrid;
  • interaction trap;
  • interaction mating