UNIT 20.4 Surface Plasmon Resonance for Measurements of Biological Interest
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Bamdad, C. 2001. Surface Plasmon Resonance for Measurements of Biological Interest. Current Protocols in Molecular Biology. 40:20.4.1–20.4.12.
- Published Online: 1 MAY 2001
- Published Print: OCT 1997
Genetic manipulations, including gene knock-outs and mutant screens, provide an initial hint as to the function of a gene product and indicate possible associated factors. To unravel complicated biological processes, which control the development of organisms, one must identify the interacting components. An in vitro technique based on an optical phenomenon, called surface plasmon resonance (SPR), can simultaneously detect interactions between unmodified proteins and directly measure kinetic parameters of the interaction. This technique is gaining popularity, due to the increased availabilty oif user-friendly machines, and an overview of the technology is presented in this unit.