Unit

UNIT 20.4 Surface Plasmon Resonance for Measurements of Biological Interest

  1. Cynthia Bamdad

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb2004s40

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Bamdad, C. 2001. Surface Plasmon Resonance for Measurements of Biological Interest. Current Protocols in Molecular Biology. 40:20.4.1–20.4.12.

Author Information

  1. Clinical Micro Sensors, Pasadena, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1997

Abstract

Genetic manipulations, including gene knock-outs and mutant screens, provide an initial hint as to the function of a gene product and indicate possible associated factors. To unravel complicated biological processes, which control the development of organisms, one must identify the interacting components. An in vitro technique based on an optical phenomenon, called surface plasmon resonance (SPR), can simultaneously detect interactions between unmodified proteins and directly measure kinetic parameters of the interaction. This technique is gaining popularity, due to the increased availabilty oif user-friendly machines, and an overview of the technology is presented in this unit.