Unit

UNIT 20.6 Identification of Protein Interactions by Far Western Analysis

  1. Diane G. Edmondson,
  2. Sharon Y. Roth

Published Online: 1 AUG 2001

DOI: 10.1002/0471142727.mb2006s55

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Edmondson, D. G. and Roth, S. Y. 2001. Identification of Protein Interactions by Far Western Analysis. Current Protocols in Molecular Biology. 55:20.6:20.6.1–20.6.10.

Author Information

  1. M.D. Anderson Cancer Center, Houston, Texas

Publication History

  1. Published Online: 1 AUG 2001
  2. Published Print: JUL 2001

Abstract

Far western blotting is a method of identifying protein-protein interactions. One protein of interest is immobilized on a solid support membrane, then probed with a non-antibody protein. Far western blots can be used to identify specific interacting proteins in a complex mixture of proteins. They are particularly useful for examining interactions between proteins that are difficult to analyze by other methods due to solubility problems or because they are difficult to express in cells. This method is performed totally in vitro, and the proteins of interest can be prepared in a variety of ways.