UNIT 20.6 Identification of Protein Interactions by Far Western Analysis
Published Online: 1 AUG 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Edmondson, D. G. and Roth, S. Y. 2001. Identification of Protein Interactions by Far Western Analysis. Current Protocols in Molecular Biology. 55:20.6:20.6.1–20.6.10.
- Published Online: 1 AUG 2001
- Published Print: JUL 2001
Far western blotting is a method of identifying protein-protein interactions. One protein of interest is immobilized on a solid support membrane, then probed with a non-antibody protein. Far western blots can be used to identify specific interacting proteins in a complex mixture of proteins. They are particularly useful for examining interactions between proteins that are difficult to analyze by other methods due to solubility problems or because they are difficult to express in cells. This method is performed totally in vitro, and the proteins of interest can be prepared in a variety of ways.