UNIT 21.1 Micrococcal Nuclease Analysis of Chromatin Structure
Published Online: 1 FEB 2005
Copyright © 2005 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Zaret, K. 2005. Micrococcal Nuclease Analysis of Chromatin Structure. Current Protocols in Molecular Biology. 69:21.1:21.1.1–21.1.17.
- Published Online: 1 FEB 2005
- Published Print: JAN 2005
This unit describes methodology for using micrococcal nuclease to investigate the presence of nucleosomes at a particular location in chromatin and to map the positions of nucleosomes at various levels of resolution. The approaches are readily adaptable to other probes of chromatin structure that cause DNA cleavage. Results obtained from such chromatin studies provide a structural view of the molecular environment of gene in their native context in cells.