Unit

UNIT 21.1 Micrococcal Nuclease Analysis of Chromatin Structure

  1. Ken Zaret

Published Online: 1 FEB 2005

DOI: 10.1002/0471142727.mb2101s69

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Zaret, K. 2005. Micrococcal Nuclease Analysis of Chromatin Structure. Current Protocols in Molecular Biology. 69:21.1:21.1.1–21.1.17.

Author Information

  1. Fox Chase Cancer Center, Philadelphia, Pennsylvania

Publication History

  1. Published Online: 1 FEB 2005
  2. Published Print: JAN 2005

Abstract

This unit describes methodology for using micrococcal nuclease to investigate the presence of nucleosomes at a particular location in chromatin and to map the positions of nucleosomes at various levels of resolution. The approaches are readily adaptable to other probes of chromatin structure that cause DNA cleavage. Results obtained from such chromatin studies provide a structural view of the molecular environment of gene in their native context in cells.