Unit

UNIT 21.6 Assembly of Nucleosomal Templates by Salt Dialysis

  1. Kyu-Min Lee,
  2. Geeta Narlikar

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb2106s54

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Lee, K.-M. and Narlikar, G. 2001. Assembly of Nucleosomal Templates by Salt Dialysis. Current Protocols in Molecular Biology. 54:21.6:21.6.1–21.6.16.

Author Information

  1. Massachusetts General Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2001

Abstract

Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made easily and in a short amount of time. This unit includes instructions for the various salt dialysis schemes (step versus gradient), which are accompanied by protocols for purification of core histones from bacteria and preparation of DNA for the nucleosomal arrays. Electrophoretic procedures to analyze the reconstituted complexes are also included.