UNIT 21.13 ChIP-chip for Genome-Wide Analysis of Protein Binding in Mammalian Cells
Published Online: 1 JUL 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Kim, T. H., Barrera, L. O. and Ren, B. 2007. ChIP-chip for Genome-Wide Analysis of Protein Binding in Mammalian Cells. Current Protocols in Molecular Biology. 79:21.13:21.13.1–21.13.22.
- Published Online: 1 JUL 2007
- Published Print: JUL 2007
ChIP-chip combines chromatin immunoprecipitation (ChIP) with microarrays (chip) to determine protein-DNA interactions occurring in living cells. The high throughput nature of this method makes it an ideal approach for identifying transcription factor targets or chromatin modification sites along the genome. unit 21.9 describes a protocol for analysis of protein-DNA interactions in yeast cells. This unit introduces an alternative protocol developed for mammalian cells. Curr. Protoc. Mol. Biol. 79:21.13.1-21.13.22. © 2007 by John Wiley & Sons, Inc.
- transcription factor;
- chromatin modifications;
- genome tiling microarrays;
- promoter arrays