Unit

UNIT 21.13 ChIP-chip for Genome-Wide Analysis of Protein Binding in Mammalian Cells

  1. Tae Hoon Kim1,
  2. Leah O. Barrera2,
  3. Bing Ren2,3

Published Online: 1 JUL 2007

DOI: 10.1002/0471142727.mb2113s79

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Kim, T. H., Barrera, L. O. and Ren, B. 2007. ChIP-chip for Genome-Wide Analysis of Protein Binding in Mammalian Cells. Current Protocols in Molecular Biology. 79:21.13:21.13.1–21.13.22.

Author Information

  1. 1

    Yale University School of Medicine, New Haven, Connecticut

  2. 2

    University of California, San Diego, La Jolla, California

  3. 3

    Ludwig Institute for Cancer Research, La Jolla, California

Publication History

  1. Published Online: 1 JUL 2007
  2. Published Print: JUL 2007

Abstract

ChIP-chip combines chromatin immunoprecipitation (ChIP) with microarrays (chip) to determine protein-DNA interactions occurring in living cells. The high throughput nature of this method makes it an ideal approach for identifying transcription factor targets or chromatin modification sites along the genome. unit 21.9 describes a protocol for analysis of protein-DNA interactions in yeast cells. This unit introduces an alternative protocol developed for mammalian cells. Curr. Protoc. Mol. Biol. 79:21.13.1-21.13.22. © 2007 by John Wiley & Sons, Inc.

Keywords:

  • ChIP-chip;
  • transcription factor;
  • chromatin modifications;
  • genome tiling microarrays;
  • promoter arrays