UNIT 21.17 Methylation-Sensitive Single-Molecule Analysis of Chromatin Structure
Published Online: 1 JAN 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Miranda, T. B., Kelly, T. K., Bouazoune, K. and Jones, P. A. 2010. Methylation-Sensitive Single-Molecule Analysis of Chromatin Structure. Current Protocols in Molecular Biology. 89:21.17:21.17.1–21.17.16.
- Published Online: 1 JAN 2010
- Published Print: JAN 2010
Methylation-sensitive single-molecule analysis of chromatin structure is a high-resolution method for studying nucleosome positioning. As described in this unit, this method allows for the analysis of the chromatin structure of unmethylated CpG islands or in vitro–remodeled nucleosomes by treatment with the CpG-specific DNA methyltransferase SssI (M.SssI), followed by bisulfite sequencing of individual progeny DNA molecules. Unlike nuclease-based approaches, this method allows each molecule to be viewed as an individual entity instead of an average population. Curr. Protoc. Mol. Biol. 89:21.17.1-21.17.16. © 2010 by John Wiley & Sons, Inc.
- chromatin remodeling;
- nucleosome positioning;
- methylation footprint