UNIT 21.23 Single Nucleotide Analysis of Cytosine Methylation by Whole-Genome Shotgun Bisulfite Sequencing
Published Online: 1 JUL 2012
Copyright © 2012 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Johnson, M. D., Mueller, M., Game, L. and Aitman, T. J. 2012. Single Nucleotide Analysis of Cytosine Methylation by Whole-Genome Shotgun Bisulfite Sequencing. Current Protocols in Molecular Biology. 99:21.23:21.23.1–21.23.28.
- Published Online: 1 JUL 2012
- Published Print: JUL 2012
Unlike other methods to assess methylation, whole-genome shotgun bisulfite sequencing is used to generate quantitative genome-wide methylation profiles at single-nucleotide resolution. As described in this unit, this method allows for the quantitative measurement of methylation at each cytosine base by treatment of genomic DNA with sodium bisulfite followed by sequencing and alignment of the reads to a reference genome. Curr. Protoc. Mol. Biol. 99:21.23.1-21.23.28. © 2012 by John Wiley & Sons, Inc.
- DNA methylation;
- bisulfite sequencing;
- second-generation sequencing;
- methylation profiling;
- read mapping