Unit

UNIT 21.23 Single Nucleotide Analysis of Cytosine Methylation by Whole-Genome Shotgun Bisulfite Sequencing

  1. Michelle D. Johnson1,
  2. Michael Mueller1,
  3. Laurence Game2,
  4. Timothy J. Aitman2

Published Online: 1 JUL 2012

DOI: 10.1002/0471142727.mb2123s99

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Johnson, M. D., Mueller, M., Game, L. and Aitman, T. J. 2012. Single Nucleotide Analysis of Cytosine Methylation by Whole-Genome Shotgun Bisulfite Sequencing. Current Protocols in Molecular Biology. 99:21.23:21.23.1–21.23.28.

Author Information

  1. 1

    National Heart & Lung Institute, Imperial College London, London, United Kingdom

  2. 2

    MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College London, London, United Kingdom

Publication History

  1. Published Online: 1 JUL 2012
  2. Published Print: JUL 2012

Abstract

Unlike other methods to assess methylation, whole-genome shotgun bisulfite sequencing is used to generate quantitative genome-wide methylation profiles at single-nucleotide resolution. As described in this unit, this method allows for the quantitative measurement of methylation at each cytosine base by treatment of genomic DNA with sodium bisulfite followed by sequencing and alignment of the reads to a reference genome. Curr. Protoc. Mol. Biol. 99:21.23.1-21.23.28. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • DNA methylation;
  • bisulfite sequencing;
  • second-generation sequencing;
  • methylation profiling;
  • read mapping