UNIT 21.27 Genome-Scale Mapping of DNase I Hypersensitivity
Published Online: 1 JUL 2013
Copyright © 2001 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
John, S., Sabo, P. J., Canfield, T. K., Lee, K., Vong, S., Weaver, M., Wang, H., Vierstra, J., Reynolds, A. P., Thurman, R. E. and Stamatoyannopoulos, J. A. 2013. Genome-Scale Mapping of DNase I Hypersensitivity. Current Protocols in Molecular Biology. 103:21.27.1–21.27.20.
- Published Online: 1 JUL 2013
- Published Print: JUL 2013
DNase I-seq is a global and high-resolution method that uses the nonspecific endonuclease DNase I to map chromatin accessibility. These accessible regions, designated as DNase I hypersensitive sites (DHSs), define the regulatory features, (e.g., promoters, enhancers, insulators, and locus control regions) of complex genomes. In this unit, methods are described for nuclei isolation, digestion of nuclei with limiting concentrations of DNase I, and the biochemical fractionation of DNase I hypersensitive sites in preparation for high-throughput sequencing. DNase I-seq is an unbiased and robust method that is not predicated on an a priori understanding of regulatory patterns or chromatin features. Curr. Protoc. Mol. Biol. 103:21.27.1–21.27.20. © 2013 by John Wiley & Sons, Inc.
- DNase I-seq;
- regulatory DNA