Unit

UNIT 22.1 Overview of DNA Microarrays: Types, Applications, and Their Future

  1. Roger Bumgarner

Published Online: 1 JAN 2013

DOI: 10.1002/0471142727.mb2201s101

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Bumgarner, R. 2013. Overview of DNA Microarrays: Types, Applications, and Their Future. Current Protocols in Molecular Biology. 101:22.1:22.1.1–22.1.11.

Author Information

  1. Department of Microbiology, University of Washington, Seattle, Washington

Publication History

  1. Published Online: 1 JAN 2013
  2. Published Print: JAN 2013

Abstract

This unit provides an overview of DNA microarrays. Microarrays are a technology in which thousands of nucleic acids are bound to a surface and are used to measure the relative concentration of nucleic acid sequences in a mixture via hybridization and subsequent detection of the hybridization events. This overview first discusses the history of microarrays and the antecedent technologies that led to their development. This is followed by discussion of the methods of manufacture of microarrays and the most common biological applications. The unit ends with a brief description of the limitations of microarrays and discusses how microarrays are being rapidly replaced by DNA sequencing technologies. Curr. Protoc. Mol. Biol. 101:22.1.1–22.1.11. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • microarrays;
  • genomics;
  • gene expression;
  • genotyping