Unit

UNIT 22.3 Profiling Human Gene Expression with cDNA Microarrays

  1. Yuan Jiang,
  2. John Lueders,
  3. Arthur Glatfelter,
  4. Chris Gooden,
  5. Michael Bittner

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb2203s53

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Jiang, Y., Lueders, J., Glatfelter, A., Gooden, C. and Bittner, M. 2001. Profiling Human Gene Expression with cDNA Microarrays. Current Protocols in Molecular Biology. 53:22.3:22.3.1–22.3.26.

Author Information

  1. National Human Genome Research Institute, NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 2001

Abstract

As a result of genomics initiatives worldwide, it has become increasingly easy to obtain cDNA clones representing the 3' ends of many human genes. This unit describes methods that allow these clones to be used as hybridization detectors in a highly parallel assay of gene expression. Protocols are provided for preparing cDNA microarrays, extracting RNA from cells of interest and preparing fluorescently labeled cDNA representations of the message pools, and hybridizing the labeled cDNAs to the microarrays.