UNIT 22.3 Profiling Human Gene Expression with cDNA Microarrays
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Jiang, Y., Lueders, J., Glatfelter, A., Gooden, C. and Bittner, M. 2001. Profiling Human Gene Expression with cDNA Microarrays. Current Protocols in Molecular Biology. 53:22.3:22.3.1–22.3.26.
- Published Online: 1 MAY 2001
- Published Print: MAY 2001
As a result of genomics initiatives worldwide, it has become increasingly easy to obtain cDNA clones representing the 3' ends of many human genes. This unit describes methods that allow these clones to be used as hybridization detectors in a highly parallel assay of gene expression. Protocols are provided for preparing cDNA microarrays, extracting RNA from cells of interest and preparing fluorescently labeled cDNA representations of the message pools, and hybridizing the labeled cDNAs to the microarrays.