UNIT 22.4 Overview of mRNA Expression Profiling Using DNA Microarrays

  1. Fumiaki Katagiri,
  2. Jane Glazebrook

Published Online: 1 JAN 2009

DOI: 10.1002/0471142727.mb2204s85

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Katagiri, F. and Glazebrook, J. 2009. Overview of mRNA Expression Profiling Using DNA Microarrays. Current Protocols in Molecular Biology. 85:22.4:22.4.1–22.4.13.

Author Information

  1. University of Minnesota, St. Paul, Minnesota

Publication History

  1. Published Online: 1 JAN 2009
  2. Published Print: JAN 2009


DNA microarray technology allows simultaneous measurement of the mRNA levels of thousands of genes. This powerful technology has applications in addressing many biological questions that were not approachable previously; however, the enormous size of microarray data sets leads to issues of experimental design and statistical analysis that are unfamiliar to many molecular biologists. The type of array used, the design of the biological experiment, the number of experimental replicates, and the statistical method for data analysis should all be chosen based on the scientific goals of the investigator. This overview presents a discussion of the relative merits and limitations of various methods with respect to some common applications of microarray experiments. Curr. Protoc. Mol. Biol. 85:22.4.1-22.4.13. © 2009 by John Wiley & Sons, Inc.


  • oligonucleotide arrays;
  • statistical analysis;
  • significance tests;
  • false discovery rate;
  • transcriptome profiling