UNIT 23.2 Mouse Embryo Fibroblast (MEF) Feeder Cell Preparation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Conner, D. A. 2001. Mouse Embryo Fibroblast (MEF) Feeder Cell Preparation. Current Protocols in Molecular Biology. 51:23.2:23.2.1–23.2.7.
- Published Online: 1 MAY 2001
- Published Print: JUL 2000
Mitotically inactive mouse embryo fibroblasts (MEFs) are commonly used as feeder layers to prevent the differentiation of mouse embryonic stem (ES) cells. This unit describes the isolation of MEFs and the use of g-irradiation or mitomycin C to produce inactivated feeders suitable for the culture of ES cells.