Unit

UNIT 23.2 Mouse Embryo Fibroblast (MEF) Feeder Cell Preparation

  1. David A. Conner

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb2302s51

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Conner, D. A. 2001. Mouse Embryo Fibroblast (MEF) Feeder Cell Preparation. Current Protocols in Molecular Biology. 51:23.2:23.2.1–23.2.7.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 2000

Abstract

Mitotically inactive mouse embryo fibroblasts (MEFs) are commonly used as feeder layers to prevent the differentiation of mouse embryonic stem (ES) cells. This unit describes the isolation of MEFs and the use of g-irradiation or mitomycin C to produce inactivated feeders suitable for the culture of ES cells.