UNIT 23.3 Mouse Embryonic Stem (ES) Cell Culture
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Conner, D. A. 2001. Mouse Embryonic Stem (ES) Cell Culture. Current Protocols in Molecular Biology. 51:23.3.1–23.3.6.
- Published Online: 1 MAY 2001
- Published Print: JUL 2000
Mouse embryonic stem (ES) cells are used to generate mouse mutants by gene targeting and blastocyst-mediated transgenesis. ES cells must be cultured under conditions that prevent differentiation to maintain their ability to transmit altered alleles by contributing to the germ line. This unit describes one of the most common methods for culturing ES cells using mouse embryo fibroblast (MEF) feeder layers and recombinant leukemia inhibitory factor (LIF) to prevent differentiation.