UNIT 23.3 Mouse Embryonic Stem (ES) Cell Culture

  1. David A. Conner

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb2303s51

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Conner, D. A. 2001. Mouse Embryonic Stem (ES) Cell Culture. Current Protocols in Molecular Biology. 51:23.3:23.3.1–23.3.6.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 2000


Mouse embryonic stem (ES) cells are used to generate mouse mutants by gene targeting and blastocyst-mediated transgenesis. ES cells must be cultured under conditions that prevent differentiation to maintain their ability to transmit altered alleles by contributing to the germ line. This unit describes one of the most common methods for culturing ES cells using mouse embryo fibroblast (MEF) feeder layers and recombinant leukemia inhibitory factor (LIF) to prevent differentiation.