UNIT 23.4 Mouse Embryonic Stem (ES) Cell Isolation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Conner, D. A. 2001. Mouse Embryonic Stem (ES) Cell Isolation. Current Protocols in Molecular Biology. 52:23.4:23.4.1–23.4.9.
- Published Online: 1 MAY 2001
- Published Print: OCT 2000
Building on the technology presented in earlier units of Chapter 23, this unit describes the basic method for the isolation of mouse embryonic (ES) stem cells. ES cells from wild-type mice may be used for the production of mouse mutants by homologous recombination and blastocyst-mediated transgenesis. ES cells from mutant mouse lines may be used in the analysis of mutant phenotypes.