Unit

UNIT 23.4 Mouse Embryonic Stem (ES) Cell Isolation

  1. David A. Conner

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb2304s52

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Conner, D. A. 2001. Mouse Embryonic Stem (ES) Cell Isolation. Current Protocols in Molecular Biology. 52:23.4:23.4.1–23.4.9.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 2000

Abstract

Building on the technology presented in earlier units of Chapter 23, this unit describes the basic method for the isolation of mouse embryonic (ES) stem cells. ES cells from wild-type mice may be used for the production of mouse mutants by homologous recombination and blastocyst-mediated transgenesis. ES cells from mutant mouse lines may be used in the analysis of mutant phenotypes.