UNIT 23.6 Production of a Homozygous Mutant Embryonic Stem Cell Line (Double Knockout)
Published Online: 1 APR 2008
Copyright © 2008 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Mortensen, R. 2008. Production of a Homozygous Mutant Embryonic Stem Cell Line (Double Knockout). Current Protocols in Molecular Biology. 82:23.6:23.6.1–23.6.4.
- Published Online: 1 APR 2008
- Published Print: APR 2008
Under some circumstances, it may be desirable to produce a mouse cell clone in which both alleles of a desired gene are mutated. This may be because the mutation causes embryonic lethality in homozygous animals, or to test cultured cells before an animal is produced. This protocol details an easy method for obtaining homozygous cells by homologous recombination without the need for two targeting events. Curr. Protoc. Mol. Biol. 82:23.6.1-23.6.4. © 2008 by John Wiley & Sons, Inc.
- homologous recombination;
- double knockout