UNIT 23.9 Transgenic Mouse Production By Zygote Injection
Published Online: 1 NOV 2004
Copyright © 2004 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Conner, D. A. 2004. Transgenic Mouse Production By Zygote Injection. Current Protocols in Molecular Biology. 68:23.9:23.9.1–23.9.29.
- Published Online: 1 NOV 2004
- Published Print: OCT 2004
This unit describes methods for the production of transgenic mice by injection of DNA into zygotes, including fertilized-egg isolation, zygote injection, and oviduct reimplantation. Methods for the preparation of plasmid and BAC DNA suitable for microinjection are also presented.
- pronuclear injection