Unit

UNIT 23.9 Transgenic Mouse Production By Zygote Injection

  1. David A. Conner

Published Online: 1 NOV 2004

DOI: 10.1002/0471142727.mb2309s68

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Conner, D. A. 2004. Transgenic Mouse Production By Zygote Injection. Current Protocols in Molecular Biology. 68:23.9:23.9.1–23.9.29.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 NOV 2004
  2. Published Print: OCT 2004

Abstract

This unit describes methods for the production of transgenic mice by injection of DNA into zygotes, including fertilized-egg isolation, zygote injection, and oviduct reimplantation. Methods for the preparation of plasmid and BAC DNA suitable for microinjection are also presented.

Keywords:

  • transgenic;
  • transgene;
  • zygote;
  • pronuclear injection