UNIT 23.15 Reprogramming of Mouse, Rat, Pig, and Human Fibroblasts into iPS Cells

  1. Kuppusamy Rajarajan1,
  2. Marc C. Engels1,
  3. Sean M. Wu1,2

Published Online: 1 JAN 2012

DOI: 10.1002/0471142727.mb2315s97

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Rajarajan, K., Engels, M. C. and Wu, S. M. 2012. Reprogramming of Mouse, Rat, Pig, and Human Fibroblasts into iPS Cells. Current Protocols in Molecular Biology. 97:23.15:23.15.1–23.15.32.

Author Information

  1. 1

    Cardiovascular Research Center, Division of Cardiology, Department of Medicine, Massachusetts General Hospital, Boston, Massachusetts

  2. 2

    Harvard Stem Cell Institute, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 JAN 2012
  2. Published Print: JAN 2012


The induction of pluripotency in somatic cells by transcription-factor overexpression has been widely regarded as one of the major breakthroughs in stem cell biology within this decade. The generation of these induced pluripotent stem cells (iPSCs) has enabled investigators to develop in vitro disease models for biological discovery and drug screening, and in the future, patient-specific therapy for tissue or organ regeneration. While new technologies for reprogramming are continually being discovered, the availability of iPSCs from different species is also increasing rapidly. Comparison of iPSCs across species may provide new insights into key aspects of pluripotency and early embryonic development. iPSCs from large animals may enable the generation of genetically modified large animal models or potentially transplantable donor tissues or organs. This unit describes the procedure for the generation of iPSCs from mouse, rat, pig and human fibroblasts. Curr. Protoc. Mol. Biol. 97:23.15.1-23.15.32. © 2012 by John Wiley & Sons, Inc.


  • induced pluripotent stem cells;
  • reprogramming;
  • fibroblasts;
  • mouse;
  • rat;
  • human;
  • pig;
  • lentivirus;
  • retrovirus