UNIT 24.2 Design, Synthesis, and Amplification of DNA Pools for Construction of Combinatorial Pools and Libraries
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Pollard, J., Bell, S. D. and Ellington, A. D. 2001. Design, Synthesis, and Amplification of DNA Pools for Construction of Combinatorial Pools and Libraries. Current Protocols in Molecular Biology. 52:24.2:24.2.1–24.2.24.
- Published Online: 1 MAY 2001
- Published Print: OCT 2000
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This unit describes the design, synthesis, and amplification of random-sequence DNA pools, from which functional nucleic acid-binding or catalytic species can be selected. Since it is an expensive and time-consuming process, the authors have provided an extensive strategic planning section to guide investigators in designing and constructing the pool.