Unit

UNIT 24.2 Design, Synthesis, and Amplification of DNA Pools for Construction of Combinatorial Pools and Libraries

  1. Jack Pollard1,
  2. Sabine D. Bell2,
  3. Andrew D. Ellington2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb2402s52

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Pollard, J., Bell, S. D. and Ellington, A. D. 2001. Design, Synthesis, and Amplification of DNA Pools for Construction of Combinatorial Pools and Libraries. Current Protocols in Molecular Biology. 52:24.2:24.2.1–24.2.24.

Author Information

  1. 1

    Mintz Levin Cohn Glovsky Ferris and Popeo, Boston, Massachusetts

  2. 2

    University of Texas, Austin, Texas

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 2000

This is not the most recent version of the article. View current version (1 OCT 2009)

Abstract

This unit describes the design, synthesis, and amplification of random-sequence DNA pools, from which functional nucleic acid-binding or catalytic species can be selected. Since it is an expensive and time-consuming process, the authors have provided an extensive strategic planning section to guide investigators in designing and constructing the pool.