UNIT 24.3 In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization
Published Online: 1 OCT 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Hall, B., Arshad, S., Seo, K., Bowman, C., Corley, M., Jhaveri, S. D. and Ellington, A. D. 2009. In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization. Current Protocols in Molecular Biology. 88:24.3:24.3.1–24.3.27.
- Published Online: 1 OCT 2009
- Published Print: OCT 2009
This unit describes the selection of aptamers from a pool of single-stranded RNA by binding to a protein target. Aptamers generated from this selection experiment can potentially act as protein function inhibitors, and may find applications as therapeutic or diagnostic reagents. A pool of dsDNA is used to generate an ssRNA pool, which is mixed with the protein target. Bound complexes are separated from unbound reagents by filtration, and the RNA:protein complexes are amplified by a combination of reverse transcription, PCR, and in vitro transcription. Curr. Protoc. Mol. Biol. 88:24.3.1-24.3.27. © 2009 by John Wiley & Sons, Inc.
- in vitro selection;
- affinity reagent;
- filter binding assay;