Unit

UNIT 24.3 In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization

  1. Bradley Hall1,
  2. Seyed Arshad2,
  3. Kyunghyun Seo2,
  4. Catherine Bowman2,
  5. Meredith Corley2,
  6. Sulay D. Jhaveri3,
  7. Andrew D. Ellington1,2

Published Online: 1 OCT 2009

DOI: 10.1002/0471142727.mb2403s88

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Hall, B., Arshad, S., Seo, K., Bowman, C., Corley, M., Jhaveri, S. D. and Ellington, A. D. 2009. In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization. Current Protocols in Molecular Biology. 88:24.3:24.3.1–24.3.27.

Author Information

  1. 1

    Department of Chemistry and Biochemistry, University of Texas, Austin, Texas

  2. 2

    Freshman Research Initiative, University of Texas, Austin, Texas

  3. 3

    Nova Research, Inc., Alexandria, Virginia

Publication History

  1. Published Online: 1 OCT 2009
  2. Published Print: OCT 2009

Abstract

This unit describes the selection of aptamers from a pool of single-stranded RNA by binding to a protein target. Aptamers generated from this selection experiment can potentially act as protein function inhibitors, and may find applications as therapeutic or diagnostic reagents. A pool of dsDNA is used to generate an ssRNA pool, which is mixed with the protein target. Bound complexes are separated from unbound reagents by filtration, and the RNA:protein complexes are amplified by a combination of reverse transcription, PCR, and in vitro transcription. Curr. Protoc. Mol. Biol. 88:24.3.1-24.3.27. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • aptamer;
  • in vitro selection;
  • affinity reagent;
  • filter binding assay;
  • SELEX