Unit

UNIT 24.4 Peptide Aptamers: Dominant “Genetic” Agents for Forward and Reverse Analysis of Cellular Processes

  1. C. Ronald Geyer

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb2404s52

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Geyer, C. R. 2001. Peptide Aptamers: Dominant “Genetic” Agents for Forward and Reverse Analysis of Cellular Processes. Current Protocols in Molecular Biology. 52:24.4:24.4.1–24.4.25.

Author Information

  1. University of Florida, Gainesville, Florida

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 2000

Abstract

Peptide aptamers are the newest in the class of “genetic” agents that aid in the analysis of cellular processes. Peptide aptamers interact with and can inactivate gene products, but don't mutate the DNA that encodes them. Reverse analysis with peptide aptamers involves isolating aptamers that interact with a specific protein and monitoring the resulting aptamer-induced phenotype. Conversely, forward analysis with peptide aptamers involves expressing combinatorial libraries of aptamers within an organism and screening for aptamer-induced variations in their phenotypes. The two-hybrid system is used in both processes to help identify the protein interactions, and variations from the basic procedure as described in unit Unavailable are presented here.