UNIT 24.4 Peptide Aptamers: Dominant “Genetic” Agents for Forward and Reverse Analysis of Cellular Processes
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Geyer, C. R. 2001. Peptide Aptamers: Dominant “Genetic” Agents for Forward and Reverse Analysis of Cellular Processes. Current Protocols in Molecular Biology. 52:24.4:24.4.1–24.4.25.
- Published Online: 1 MAY 2001
- Published Print: OCT 2000
Peptide aptamers are the newest in the class of “genetic” agents that aid in the analysis of cellular processes. Peptide aptamers interact with and can inactivate gene products, but don't mutate the DNA that encodes them. Reverse analysis with peptide aptamers involves isolating aptamers that interact with a specific protein and monitoring the resulting aptamer-induced phenotype. Conversely, forward analysis with peptide aptamers involves expressing combinatorial libraries of aptamers within an organism and screening for aptamer-induced variations in their phenotypes. The two-hybrid system is used in both processes to help identify the protein interactions, and variations from the basic procedure as described in unit Unavailable are presented here.