Unit

UNIT 25A.3 Laser Microdissection-Mediated Isolation and In Vitro Transcriptional Amplification of Plant RNA

  1. Michael J. Scanlon1,
  2. Kazuhiro Ohtsu2,
  3. Marja C.P. Timmermans3,
  4. Patrick S. Schnable2

Published Online: 1 JUL 2009

DOI: 10.1002/0471142727.mb25a03s87

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Scanlon, M. J., Ohtsu, K., Timmermans, M. C. and Schnable, P. S. 2009. Laser Microdissection-Mediated Isolation and In Vitro Transcriptional Amplification of Plant RNA. Current Protocols in Molecular Biology. 87:A:25A.3:25A.3.1–25A.3.15.

Author Information

  1. 1

    Cornell University, Ithaca, New York

  2. 2

    Iowa State University, Ames, Iowa

  3. 3

    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

Publication History

  1. Published Online: 1 JUL 2009
  2. Published Print: JUL 2009

This is not the most recent version of the article. View current version (1 OCT 2015)

Abstract

Protocols for laser microdissection and linear amplification of RNA from fixed, sectioned plant tissues are described. When combined with quantitative RT-PCR, microarray analysis, or RNA-sequencing, these procedures enable quantitative analyses of transcript accumulation from microscopic quantities of specific plant organs, tissues, or single cells. Curr. Protoc. Mol. Biol. 87:25A.3.1-25A.3.15 © 2009 by John Wiley & Sons, Inc.

Keywords:

  • laser microdissection;
  • plants;
  • RNA amplification;
  • transcriptomics