UNIT 25B.3 Differential Display of mRNA by PCR
Published Online: 1 NOV 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Liang, P. and Pardee, A. B. 2001. Differential Display of mRNA by PCR. Current Protocols in Molecular Biology. 56:B:25B.3:25B.3.1–25B.3.10.
- Published Online: 1 NOV 2001
- Published Print: OCT 2001
Formerly unit Unavailable , this unit describes how differential display techniques allow the identification and subsequent isolation of differentially expressed genes that requires no knowledge of sequences, but rather PCR amplification using arbitrary oligonucleotides and high resolution polyacrylamide gel electrophoresis.