Unit

UNIT 25B.3 Differential Display of mRNA by PCR

  1. Peng Liang1,
  2. Arthur B. Pardee2

Published Online: 1 NOV 2001

DOI: 10.1002/0471142727.mb25b03s56

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Liang, P. and Pardee, A. B. 2001. Differential Display of mRNA by PCR. Current Protocols in Molecular Biology. 56:B:25B.3:25B.3.1–25B.3.10.

Author Information

  1. 1

    Vanderbilt-Ingram Cancer Center, Nashville, Tennessee

  2. 2

    Dana Farber Cancer Institute, Boston, Massachusetts

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: OCT 2001

Abstract

Formerly unit Unavailable , this unit describes how differential display techniques allow the identification and subsequent isolation of differentially expressed genes that requires no knowledge of sequences, but rather PCR amplification using arbitrary oligonucleotides and high resolution polyacrylamide gel electrophoresis.