UNIT 25B.4 Restriction-Mediated Differential Display (RMDD)
Published Online: 1 NOV 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Fischer, A. 2001. Restriction-Mediated Differential Display (RMDD). Current Protocols in Molecular Biology. 56:B:25B.4:25B.4.1–25B.4.17.
- Published Online: 1 NOV 2001
- Published Print: OCT 2001
A variation on differential display, restriction-mediated differential display (RMDD) presents an alternative approach to the fragment display technologies. It touts high sensitivity (detection of mRNAs at a dilution of 1:100,000 and of regulation factors lower than two-fold), a strategy for avoiding false positives, nonradioactive detection, and universal applicability to any polyadenylated RNA.