UNIT 25B.4 Restriction-Mediated Differential Display (RMDD)

  1. Achim Fischer

Published Online: 1 NOV 2001

DOI: 10.1002/0471142727.mb25b04s56

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Fischer, A. 2001. Restriction-Mediated Differential Display (RMDD). Current Protocols in Molecular Biology. 56:B:25B.4:25B.4.1–25B.4.17.

Author Information

  1. F. Hoffmann-La Roche AG, Basel, Switzerland

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: OCT 2001


A variation on differential display, restriction-mediated differential display (RMDD) presents an alternative approach to the fragment display technologies. It touts high sensitivity (detection of mRNAs at a dilution of 1:100,000 and of regulation factors lower than two-fold), a strategy for avoiding false positives, nonradioactive detection, and universal applicability to any polyadenylated RNA.