Unit

UNIT 25B.6 Serial Analysis of Gene Expression (SAGE)

  1. Seth Blackshaw,
  2. Jae B. Kim,
  3. Brad St. Croix,
  4. Kornelia Polyak

Published Online: 1 FEB 2003

DOI: 10.1002/0471142727.mb25b06s61

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Blackshaw, S., Kim, J. B., Croix, B. S. and Polyak, K. 2003. Serial Analysis of Gene Expression (SAGE). Current Protocols in Molecular Biology. 25:B:25B.6.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 FEB 2003
  2. Published Print: JAN 2003

This is not the most recent version of the article. View current version (1 OCT 2007)

Abstract

Serial Analysis of Gene Expression (SAGE) involves the generation of short fragments of DNA, or tags, from a defined point in the sequence of all cDNAs in the sample analyzed. This short tag, because of its defined presence in the sequence, is typically sufficient to uniquely identify every transcript in the sample. A comprehensive profile of gene expression in any sample can thus be generated. Absolute, rather than relative, measurements of RNA abundance levels are made, which will be useful in the reliable comparison of data between laboratories.Serial Analysis of Gene Expression (SAGE) involves the generation of short fragments of DNA, or tags, from a defined point Serial Analysis of Gene Expression (SAGE) involves the generation of short fragments of DNA, or tags, from a defined point