Unit

UNIT 26.2 Gene Silencing by RNAi in Mammalian Cells

  1. Matthias John1,
  2. Anke Geick1,
  3. Philipp Hadwiger1,
  4. Hans-Peter Vornlocher1,
  5. Olaf Heidenreich2

Published Online: 1 MAY 2003

DOI: 10.1002/0471142727.mb2602s62

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

John, M., Geick, A., Hadwiger, P., Vornlocher, H.-P. and Heidenreich, O. 2003. Gene Silencing by RNAi in Mammalian Cells. Current Protocols in Molecular Biology. 62:26.2:26.2.1–26.2.14.

Author Information

  1. 1

    Ribopharma AG, Kulmbach, Germany

  2. 2

    University of Tübingen, Tübingen, Germany

Publication History

  1. Published Online: 1 MAY 2003
  2. Published Print: APR 2003

This is not the most recent version of the article. View current version (1 JUL 2015)

Abstract

This unit provides information how to use short interfering RNA (siRNA) for sequence specific gene silencing in mammalian cells. Several ways for siRNA generation and optimisation, as well as recommendations for cell transfection are presented.