UNIT 26.2 Gene Silencing by RNAi in Mammalian Cells
Published Online: 1 MAY 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
John, M., Geick, A., Hadwiger, P., Vornlocher, H.-P. and Heidenreich, O. 2003. Gene Silencing by RNAi in Mammalian Cells. Current Protocols in Molecular Biology. 62:26.2:26.2.1–26.2.14.
- Published Online: 1 MAY 2003
- Published Print: APR 2003
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This unit provides information how to use short interfering RNA (siRNA) for sequence specific gene silencing in mammalian cells. Several ways for siRNA generation and optimisation, as well as recommendations for cell transfection are presented.