Unit

UNIT 26.6 RNAi in Transgenic Plants

  1. Yanhai Yin1,
  2. Joanne Chory2,
  3. David Baulcombe3

Published Online: 1 NOV 2005

DOI: 10.1002/0471142727.mb2606s72

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Yin, Y., Chory, J. and Baulcombe, D. 2005. RNAi in Transgenic Plants. Current Protocols in Molecular Biology. 72:26.6:26.6.1–26.6.19.

Author Information

  1. 1

    Iowa State University, Ames, Iowa

  2. 2

    The Salk Institute for Biological Studies, La Jolla, California

  3. 3

    John Innes Centre, Norwich, United Kingdom

Publication History

  1. Published Online: 1 NOV 2005
  2. Published Print: OCT 2005

Abstract

RNA silencing in plants is a rapid and facile approach for assignment of gene function and virus resistance. It allows selective RNA degradation through a mechanism that is given specificity by short interfering RNAs. The RNA silencer sequences are normally delivered as transgenes or a part of virus-vectors. This unit provides Basic Protocols for transgene and virus induced silencing. It also includes a Basic Protocol for protein overexpression using viral proteins that suppress silencing.

Keywords:

  • double stranded RNA;
  • posttranscriptional control;
  • antisense RN