Unit

UNIT 26.7 Analysis of Small Endogenous RNAs

  1. Nelson C. Lau

Published Online: 1 JAN 2008

DOI: 10.1002/0471142727.mb2607s81

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Lau, N. C. 2008. Analysis of Small Endogenous RNAs. Current Protocols in Molecular Biology. 81:26.7:26.7.1–26.7.15.

Author Information

  1. Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 JAN 2008
  2. Published Print: JAN 2008

Abstract

Eukaryotic cells express small noncoding RNAs to silence target genes or regulate chromatin domains. MicroRNAs (miRNA) are endogenous small RNAs that are thought to each regulate multiple mRNA targets. To gain a deeper understanding of processes regulated by small noncoding RNAs, techniques are required to identify and detect them. This unit describes standard laboratory methods for and comments about high-throughput technologies for the identification and detection of small RNAs. This unit also outlines a cell-based reporter gene assay for assessing the regulatory potential of a microRNA on a candidate mRNA target. Curr. Protoc. Mol. Biol. 81:26.7.1-26.7.15. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • Small RNA;
  • RNAi;
  • miRNA;
  • microRNA regulation;
  • gene silencing