Unit

UNIT 27.1 Agarose Gel Separation/Isolation of RNA-Protein Complexes

  1. Chung-Sheng Brian Lee,
  2. Rita Das,
  3. Robin Reed

Published Online: 1 AUG 2003

DOI: 10.1002/0471142727.mb2701s63

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Lee, C.-S. B., Das, R. and Reed, R. 2003. Agarose Gel Separation/Isolation of RNA-Protein Complexes. Current Protocols in Molecular Biology. 63:27.1:27.1.1–27.1.5.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: JUL 2003

Abstract

In many methods currently used to analyze RNA-protein complexes, high salt or other stringent treatments are required for reducing nonspecific interactions and resolving the complex of interest. RNA-protein complexes often dissociate on native polyacrylamide gels and can only be detected on density gradients or by gel filtration. Agarose gel electrophoresis provides an alternative method that is simple, rapid, and can have high resolution of RNA-protein complexes. Moreover, the use of low-melting point agarose for the fractionation readily allows for the isolation of the RNA species in each complex detected on the native gel.

Keywords:

  • RNA-protein complex;
  • low-melting-point agarose;
  • agarose minigel system