Unit

UNIT 27.2 Identification of RNA Binding Proteins by UV Cross-Linking

  1. Ming-Juan Luo,
  2. Robin Reed

Published Online: 1 AUG 2003

DOI: 10.1002/0471142727.mb2702s63

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Luo, M.-J. and Reed, R. 2003. Identification of RNA Binding Proteins by UV Cross-Linking. Current Protocols in Molecular Biology. 63:27.2.1–27.2.10.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: JUL 2003

Abstract

One of the major focuses of modern biology is to understand the dynamics of RNA-protein interactions, including factors that interact with mRNA, rRNA, tRNA, snRNA, hnRNA, siRNA, and viral RNA. Identification the direct interactions between proteins and RNA has greatly advanced our knowledge about the function of RNA-protein machines. UV crosslinking is a straightforward and powerful tool in this endeavor.