UNIT 27.2 Identification of RNA Binding Proteins by UV Cross-Linking
Published Online: 1 AUG 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Luo, M.-J. and Reed, R. 2003. Identification of RNA Binding Proteins by UV Cross-Linking. Current Protocols in Molecular Biology. 63:27.2:27.2.1–27.2.10.
- Published Online: 1 AUG 2003
- Published Print: JUL 2003
One of the major focuses of modern biology is to understand the dynamics of RNA-protein interactions, including factors that interact with mRNA, rRNA, tRNA, snRNA, hnRNA, siRNA, and viral RNA. Identification the direct interactions between proteins and RNA has greatly advanced our knowledge about the function of RNA-protein machines. UV crosslinking is a straightforward and powerful tool in this endeavor.