Unit

UNIT 28.2 Isolation and Immortalization of Lymphocytes

  1. Paul D. Ling,
  2. Helen M. Huls

Published Online: 1 MAY 2005

DOI: 10.1002/0471142727.mb2802s70

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Ling, P. D. and Huls, H. M. 2005. Isolation and Immortalization of Lymphocytes. Current Protocols in Molecular Biology. 70:28.2.1–28.2.5.

Author Information

  1. Baylor College of Medicine, Houston, Texas

Publication History

  1. Published Online: 1 MAY 2005
  2. Published Print: APR 2005

Abstract

Recent advances in Genomics and Proteomics have necessitated a constant supply of DNA derived from specific genotypes. Lymphoblastoid cell lines (LCLs) are of great practical value as an unlimited source of stable genomic DNA and viable cells, which can be used to perform a variety of biochemical and molecular studies. LCLs are typically generated by infection of primary lymphocytes with Epstein-Barr virus (EBV). In this unit, we describe simple procedures for production of EBV, isolation of lymphocytes, EBV infection of lymphocytes, and isolation of the resulting LCLs.

Keywords:

  • B lymphocyte;
  • Epstein-Barr virus;
  • Immortalization;
  • Lymphoblastoid cell line (LCL)