Unit

UNIT 28.3 Establishment and Culture of Human Skin Fibroblasts

  1. Jacob Villegas,
  2. Michael McPhaul

Published Online: 1 AUG 2005

DOI: 10.1002/0471142727.mb2803s71

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Villegas, J. and McPhaul, M. 2005. Establishment and Culture of Human Skin Fibroblasts. Current Protocols in Molecular Biology. 71:28.3:28.3.1–28.3.9.

Author Information

  1. University of Texas Southwestern Medical Center, Dallas, Texas

Publication History

  1. Published Online: 1 AUG 2005
  2. Published Print: JUL 2005

Abstract

The establishment of skin fibroblast strains provides a vehicle by which biochemical and genetic studies may be applied. In addition to providing genetic material to study the basis of disease, fibroblast cell lines established from skin biopsies may provide the investigator with a model in which to study specific disease states or normal physiology. This unit provides methods for the biopsy, establishment, and culture maintenance of skin fibroblasts to be used in further scientific study.

Keywords:

  • Skin fibroblast;
  • Patient-derived primary culture;
  • Tissue culture